FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117705767
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTCGCGGCCCTCCCCCGCCCCACCCCGCGCGCGCCGCTCGCTCCC1252960.10644848013266843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC275750.029.7644581
TGCGACA396500.027.4678156
AGGGCGA630300.027.0853354
GCGACAT404700.027.0417967
TACCGTA209350.025.6030969
GGGCGAT256350.023.48261
TCAATCG207300.023.3718973
GGCGATC267950.022.9155272
TGGCGCG890600.022.0036134
CTACCGT256400.021.1873668
CGGAGGG837100.021.092621
ACTACCG257200.020.5068667
AAGGGTC1036300.020.049896145
GTGGCGC1128550.019.9410783
CGACATC558150.019.8434018
ACGCCCG855650.019.8158596
GCGAGGC862050.019.768317
CGGTGGC1424600.019.2153281
AATCGCC431450.019.1079222
GCTGCGA578300.019.0460534
Produced by FastQC (version 0.11.5)