FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121513475
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1574330.1295601166866473No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1558410.1282499739226452No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT1513780.12457713023185288No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1284360.10569691962146585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1635900.031.1556551
TGGCGCG1188900.030.340014
GTGGCGC1501300.025.110833
GGCGCGT1448800.023.8069925
GGTGGCG2008200.022.4626462
TGCGACA312550.021.4822546
GCGACAT323050.021.132687
CGTGCCT1877350.019.0083249
GCGTGCC1782200.018.9351678
AGGGCGA619250.018.7224524
CAATCGC191900.017.3517131
TACCGTA163200.017.3302159
GCGCGTG2099100.016.5447226
CGCGTGC2083250.016.226057
CGACATC456750.015.1383618
TCAATCG145650.015.0863573
GGGGACC6132550.014.811452145
GGGCGAT256450.014.0288871
CGTCGTT146450.013.744963145
CGGAGGG880800.013.600821
Produced by FastQC (version 0.11.5)