FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106413490
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1252760.11772567556989251No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1121590.10539923086819161No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1097560.10314105852556853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG183650.030.0059973
CAATCGC256250.028.5870721
CGGTGGC1873700.027.2784981
TGCGACA354350.022.4956656
TGGCGCG1314450.022.2794844
GCGACAT370350.021.8797057
AGGGCGA729800.019.7886984
GGGCGAT249200.019.641
GGCGATC258200.019.1835612
GTGGCGC1629100.018.8393363
GGGGACC5369750.017.644089145
TACCGTA166850.017.6042849
AATCGCC418850.017.4762612
CGGAGGG884500.016.9763131
GGCGCGT1643650.016.8673045
CGACCGT87000.016.3253231
ATATCGG200100.016.188358145
CGTGCCT1939500.016.0408699
CGACATC527700.015.7591218
GGTGGCG2551300.015.531522
Produced by FastQC (version 0.11.5)