FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114459589
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG189000.028.424623
AGGGCGA768000.028.168034
CAATCGC274700.027.3830031
TGCGACA353550.025.0750456
GCGACAT361750.024.9572167
TGGCGCG1049450.022.9758744
CGGAGGG993800.022.129331
CGGTGGC1782400.020.5856291
GGGCGAT268700.018.757951
TACCGTA192350.018.5132799
GTGGCGC1385600.018.231013
GGCGATC274750.018.005742
CGACATC511600.017.7205858
AATCGCC429750.017.588672
GGGCGAG1265950.017.556835
GAGGGCG1278100.017.3632143
GCGAGGC1249650.016.9829257
GGCGAGG1350450.016.5045726
CTACCGT218750.016.1648378
GCTGCGA567150.016.023124
Produced by FastQC (version 0.11.5)