FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166944726
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2917730.17477221772193033No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2212050.13250193959406661No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1799810.10780873664736196No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1753340.10502518060977858No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1747260.10466098821235No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1703040.10201220732184135No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1690610.1012676495093352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG912050.050.10389145
ACCGCGT366600.049.240186
ACCGCTA369400.044.8757862
CTCGCTA755150.044.0628171
CCGCGTT417150.043.198327
AGACCGC435200.040.9086234
TCGCTAT829250.039.7165572
CAGACCG489150.037.1300243
CGCTATG906400.036.287623
CCGCTAA528100.033.3593983
GCGTTCT638850.028.1190959
CGCTAAG629900.027.7868754
CGATCCT1742000.027.13071145
CACCGCT721850.024.3060281
GTGCTCT1384350.024.12262145
CTTCGAC1217400.021.973127145
GTCGCGT93000.021.8715361
CGTATCG158500.020.8700643
CGCTAGA470800.020.8412651
GCTATGT1623950.020.677134
Produced by FastQC (version 0.11.5)