FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106892744
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1069170.10002269190507448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG502850.043.19212145
ACCGCGT260200.037.7373546
CTCGCTA335750.037.6386151
TCGCTAT350050.035.2741662
CGCTATG363250.033.7612273
CTTCGAC758100.032.298412145
CCGCGTT304250.032.294887
AGACCGC298200.032.1711274
CAGACCG341850.028.8347723
ACCGCTA179150.027.4557273
CCGCTAA222550.023.6421093
TCGCGTC388750.022.8154939
CTCGCGT389200.022.6196778
CCGGTAA143150.021.0697941
CGCTAAG242600.020.9000074
CGTATCG118650.020.7833143
CGGTGCT487050.020.479156145
GCGTTCT476350.020.4055949
CGCTAGA374650.020.277451
CGATCCT761650.020.17284145
Produced by FastQC (version 0.11.5)