FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106892744
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA742250.038.8311464
CAATCGC408050.035.002731
TACCGTA187150.027.8127129
AATCGCC550700.026.0456222
TCAATCG277650.025.7034443
CGGAGGG1173650.025.60051
GCGAGGC1169300.024.670787
GGGCGAG1239000.024.2203275
CTACCGT220250.023.572938
GGCGATC271800.022.9811862
GGGCGAT273200.022.9670261
CTCGCGT151400.022.5128658
GGCGAGG1333950.022.511196
TCGCGTC152550.022.4297529
GCGACAT341450.022.4030467
GAGGGCG1328450.022.3389683
TGCGACA352300.021.4122356
ACTACCG248100.021.2738297
CGAGGCC1549050.018.9246928
TGGCGCG802750.017.6806684
Produced by FastQC (version 0.11.5)