FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145530875
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT3457860.23760318901401506No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT3272710.22488080278497605No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT3001830.2062675703695178No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2632740.1809059417803954No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2318850.15933732275024115No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2029100.1394274582627226No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1935170.13297315775776103No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1870260.12851293582890916No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1615650.11101767923816852No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1613130.11084452010612868No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1573880.10814749791066673No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1554170.10679314612792647No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1546460.10626336164061406No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1497110.10287232863816699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG771100.044.058895145
CTCGCTA1083550.042.1485941
TCGCTAT1141050.040.029972
ACCGCGT343500.037.7627646
CGCTATG1229900.037.3057373
CCGCGTT381850.034.0381437
CTTCGAC1115700.031.196583145
AGACCGC429650.029.9085184
CAGACCG453800.028.8938793
GTCTCGC1035150.028.0275964
CGATCCT2529950.025.94787145
ACCGCTA178750.024.4096032
GCTATGT1904050.024.306954
CGCGTTC547450.023.5625118
TCTCGCT1260250.023.0783215
CTATGTT2086600.022.3633675
GCGTTCT603050.021.5311249
CCGGTAA185350.020.8661821
ATGTTGC2328600.020.2300227
CCGCTAA242700.020.094443
Produced by FastQC (version 0.11.5)