FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145530875
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT1662280.11422181032031863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC2272450.037.313261
TGGCGCG1631000.032.485094
GTGGCGC2044200.029.0626663
GGTGGCG2569650.025.6327842
GGGCGAT389300.025.5764121
GGCGCGT1938700.024.2809225
GGCGATC433150.023.2619252
AGGGCGA807750.022.8956264
TGCGACA511050.022.6749066
GCGACAT535750.021.813617
GGGGACC7632300.021.644165145
CGTGCCT2376050.020.8706789
CGATCTG498400.020.7770774
CGACCGT117400.020.1878991
CAATCGC252250.020.1035981
TACCGTA203000.020.0932319
GCGTGCC2330050.019.7472388
GCGCGTG2638550.017.9201776
CGCGTGC2616450.017.6658887
TCAATCG185700.017.5871733
Produced by FastQC (version 0.11.5)