FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150154475
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2783390.18536843474028997No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2640540.17585489876342347No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2012710.13404262510324785No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1637370.1090457011021483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT596950.061.6803976
AGACCGC649100.056.5795254
CCGCGTT653850.056.370567
TCGACCG1021050.053.18286145
CAGACCG710350.052.272943
GCGTTCT901650.040.8643049
GTGCTCT1507150.038.67851145
CTCGCTA460450.035.9509661
CGCGTTC1083900.033.9003228
TCGCTAT487800.033.368592
ACCGCTA220150.031.3272362
GACCGCG1198750.030.8257265
CGCTATG525750.030.6880663
TCAGACC1540800.025.6329292
CCGCTAA301600.024.732023
CTTCGAC1362150.024.465012145
CTCAGAC1921500.023.5422291
CGATCCT1123400.023.219425145
CCGGTAA181900.021.6676121
CGCTAAG352500.020.5586764
Produced by FastQC (version 0.11.5)