FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150154475
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1681420.11197934660289012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG247850.030.3466113
TGCGACA569300.026.921656
GCGACAT583200.026.6266427
CAATCGC367300.025.9212571
AGGGCGA979400.023.8124284
GGGCGAT358400.023.4071251
GGCGATC363700.022.8107072
CGGAGGG1169950.020.4894541
CGACATC769100.020.3221158
GCTGCGA818750.018.9049224
CTGCGAC859550.018.2497795
AATCGCC583750.016.5409472
TACCGTA260300.016.1149359
GGGCGAG1521050.015.8495495
GCGAGGC1471300.015.7542787
GGTCACG820550.015.687634145
CGGCCGC4060550.015.544781145
GGCGAGG1614000.014.9876616
GAGGGCG1628700.014.672963
CGACCGT136550.014.6098291
Produced by FastQC (version 0.11.5)