FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91204308
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1888400.2070516230439466No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1688320.18511406281378728No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1522410.16692303613552992No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1454460.1594727301697196No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1441470.15804845534270157No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1089520.119459269402055No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1089170.11942089402180432No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1076310.11801087290745083No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1020340.11187410138564946No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG989450.10848719996866814No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC983660.10785236153537835No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC937060.10274295376485944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT382500.052.3549466
AGACCGC402200.049.2382624
CCGCGTT419700.047.7448927
TCGACCG500900.047.63959145
CTCGCTA570200.044.918471
CAGACCG448350.044.864023
TCGCTAT601750.042.5305442
GCGTTCT490250.040.6096769
CGCTATG642000.039.762553
ACCGCTA168100.032.5720252
GTGCTCT899100.029.727694145
CTTCGAC667050.029.2804145
GTGTGCG445600.026.683493145
GCTATGT1006200.025.7439234
GTCGCGT60550.025.4101371
CGCGTTC804850.024.6315048
CGATCCT1338300.024.315552145
CCGCTAA239650.024.1009333
TCAGACC918150.023.041312
GACCGCG871550.023.0292385
Produced by FastQC (version 0.11.5)