FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91204308
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1164120.1276387075926282No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCGCGCTTCCCTCCGCAC923620.10126933916323339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA785450.042.8759544
CAATCGC276050.033.0422251
GCGAGGC1158800.029.1124467
GGGCGAG1191150.029.0899495
CGGAGGG1203750.029.018591
TGGCGCG903500.028.1297114
CGGTGGC1620150.028.1293351
TCAATCG185850.027.9582843
GGCGAGG1284250.027.1126046
TGCGACA313800.026.3067746
GCGACAT318750.026.2452627
GAGGGCG1339150.025.9433773
GGGCGAT226050.024.8602921
AATCGCC376800.024.4147172
GGCGATC243000.023.0997242
GTGGCGC1195650.022.9675143
CGAGGCC1508050.022.4521148
TTAACGG103650.020.8042413
GGGGACC4059200.020.342514145
CGACATC425850.019.7514218
Produced by FastQC (version 0.11.5)