FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156197697
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1725020.11043824801078854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC369250.027.9958721
TCAATCG271050.025.8277573
TGGCGCG1416550.024.2874984
CGGTGGC2135350.023.9082151
GGCGCGT1509750.020.7971235
GTGGCGC1778200.019.9768283
GGGGACC5574300.019.051638145
GGTCACG817150.018.156988145
AATCGCC577000.017.9476382
CGTGCCT1979400.017.7381929
AGGGCGA846900.017.5581384
TGCGACA345350.017.4040036
GCGACAT361350.017.0318747
TACCGTA232000.016.1649279
GCGTGCC1962550.015.7977348
ACGCCCG908200.015.7384446
CGACCGT121650.014.7718361
GGTGGCG2657050.014.5689662
CGGAGGG1064850.014.51417351
CGCGTGC2263000.013.7114627
Produced by FastQC (version 0.11.5)