FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83560047
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1859280.22250825205974334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1264960.15138335190261443No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1220360.1460458728559595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT377150.063.7792476
CCGCGTT394700.060.8798567
AGACCGC400700.059.6869664
CAGACCG422700.056.940613
TCGACCG468650.053.24405145
GTGCTCT867350.051.231903145
GCGTTCT500350.047.924769
CTCGCTA278900.040.1958161
TCGCTAT297900.036.9594732
ACCGCTA125450.033.7060932
CGCTATG327300.033.6559643
CGCGTTC829900.028.7883288
TCAGACC906750.027.9727612
CCGCTAA172350.026.8461073
GACCGCG921700.026.308545
CTCAGAC1095950.025.8845461
CTTCGAC606500.023.86255145
CGCTAAG209150.021.3004344
CGATCCT466500.020.839485145
CGCTAGA346500.020.5589451
Produced by FastQC (version 0.11.5)