FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83560047
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1067170.1277129487492988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA585600.031.4437394
TCAATCG117250.028.178753
CAATCGC173550.026.8889541
CGGAGGG721550.025.9403271
GCGACAT236300.025.8482347
TGCGACA235950.025.7266186
GAGGGCG870450.021.4583023
CGACATC312100.020.0340148
GGGCGAG991400.019.0855335
GCGAGGC976300.018.6462127
GGCGAGG1019950.018.5512986
TACCGTA108600.018.0131029
AATCGCC268350.017.6200682
GGGCGAT174300.017.27661
CTGCGAC358500.017.2129465
GCTGCGA360050.017.1636494
GGCGATC181300.016.564942
CGAGGCC1164600.015.7988138
CTACCGT129650.014.9435428
CGTTCTT449750.014.737335145
Produced by FastQC (version 0.11.5)