FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143126038
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG540400.044.776257145
CTCGCTA492300.037.5416531
TCGCTAT511700.035.637142
CGCTATG513900.035.2083633
ACCGCGT303900.028.9218446
CTTCGAC840350.028.170567145
CCGCGTT316800.027.82637
AGACCGC370200.023.4928074
CAGACCG407700.022.0119113
ACCGCTA217700.020.3540673
CGTATCG136700.019.7767873
CGATCCT1156050.019.667292145
CCCGTAT264950.018.935111
CGCGTTC461250.018.8975588
CCGCTAA250300.018.887954
GACCGCG499750.017.6930435
GCGTTCT507650.017.4300549
CTCCGAT378050.017.0241181
CGCTAAG270950.016.6940425
CGGTGCT692400.015.730597145
Produced by FastQC (version 0.11.5)