FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143126038
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA764900.024.5839484
TGGCGCG933450.022.451634
CAATCGC303400.022.1363951
GGCGATC349700.021.3307552
TGCGACA431200.020.5225646
GCGACAT439800.020.4065597
GGGCGAT376550.020.1009981
CGGCCGC2214950.020.077639145
CGGTGGC1700150.019.8810621
TCAATCG241450.018.0541653
TACCGTA206150.018.0041589
GGGGACC3735850.017.619709145
GCGAGGC1064400.017.5398757
GGGCGAG1125450.017.2808325
GTGGCGC1309900.017.128453
CGATCTG469400.016.5897394
GGCGCGT1149100.016.2010365
GGCGAGG1199350.016.1664876
CGGAGGG1375950.015.0307621
CTACCGT258200.015.0141838
Produced by FastQC (version 0.11.5)