FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90131454
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA150600.026.0294729
TGCGACA275450.024.3299776
AGGGCGA557050.023.905824
GCGACAT288050.023.7145927
CAATCGC254600.023.0031131
TGGCGCG865100.022.9029454
TCAATCG191450.022.7463723
CTACCGT190800.020.8837978
CTCGCGT175300.020.1842468
TCGCGTC176650.019.8473879
CGGTGGC1419950.019.1360551
GTGGCGC1074100.018.886233
GGCGATC242000.017.9727732
GGGCGAT246550.017.8882941
ACTACCG228400.017.4180157
CGTGCCT1132550.016.9749769
CGACATC407900.016.8731088
GGGGACC3151100.016.83696145
GGTCACG446400.016.81284145
GGCGCGT1085850.016.8056035
Produced by FastQC (version 0.11.5)