FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129679459
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2766290.21331751545940672No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2629300.20275377613967377No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1665370.1284220348266567No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1453700.11209948061242297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT609800.055.01916
AGACCGC654750.050.8823664
CCGCGTT663350.050.5495837
CAGACCG719150.047.032053
GTGCTCT1462150.045.18426145
TCGACCG570950.045.169777145
GCGTTCT788250.042.516329
CTCGCTA565300.036.2312661
GACCGCG1001550.033.480115
CGCGTTC1006600.033.269148
TCGCTAT617200.032.760442
ACCGCTA196500.032.093032
CGCTATG659500.030.6438663
CTTCGAC767200.026.871515145
TCAGACC1388700.025.3331342
CCGCTAA297850.023.8510253
CGATCCT1250650.023.31003145
CTCAGAC1700000.023.2731151
CGGTGCT1035150.021.203777145
TCGCGTC239250.020.9947649
Produced by FastQC (version 0.11.5)