FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129679459
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2152930.1660193539209629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG199050.032.4127923
CAATCGC295750.030.0816541
AGGGCGA857150.027.7617784
CGGAGGG1011700.024.068131
GCGACAT370900.020.7438857
TGGCGCG997450.020.7133544
TGCGACA366450.020.6724226
AATCGCC451200.019.8487052
GGGCGAG1290850.019.0807515
GCGAGGC1250350.018.9825277
CGGTGGC1735250.018.9761771
CGGCCGC3056650.018.440296145
GGCGAGG1345750.018.292386
GGGCGAT281900.017.8664721
GGCGATC286300.017.6417582
GGGGACC3905650.017.599386145
GAGGGCG1411250.017.162493
ACGCCCG819150.017.0987266
GGTCACG751600.016.84482145
GTGGCGC1392600.016.5390243
Produced by FastQC (version 0.11.5)