FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133471449
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1999610.14981556092943893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA831600.028.5404434
TCAATCG203450.023.6575813
TGGCGCG1181750.023.3557834
CAATCGC289800.022.9907111
CGGAGGG1061500.022.9785671
GCGACAT383900.022.7979587
TGCGACA383050.022.6201216
CGGTGGC1906150.021.5664961
GGGCGAT276550.019.2829931
TACCGTA202450.019.218099
GTGGCGC1561100.018.8220883
GGGCGAG1309750.018.7112875
GAGGGCG1291750.018.649573
GGCGATC296650.018.027472
GGGGACC4881150.017.87684145
GCGAGGC1344700.017.7389897
GGCGAGG1383050.017.7369826
CGACATC507600.017.5127098
CTACCGT237600.016.925168
GCTGCGA577400.015.3444194
Produced by FastQC (version 0.11.5)