FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134752089
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2298950.17060588945674898No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1965220.1458396685783476No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1459930.10834191965662215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT533800.056.6104856
AGACCGC568550.052.7296524
CCGCGTT581300.051.952167
CAGACCG640250.047.402813
TCGACCG467400.046.70382145
GCGTTCT737300.040.9428989
CTCGCTA477200.037.681681
GTGCTCT1344650.036.9764145
TCGCTAT521600.034.1377942
CGCGTTC932600.032.220358
ACCGCTA192150.032.0259062
CGCTATG562200.031.6576063
GACCGCG1069400.028.3164655
CCGCTAA275050.024.5001563
CGATCCT1115650.024.088459145
CTTCGAC680000.024.034193145
TCAGACC1406050.022.7298722
CTCAGAC1741050.021.292741
CGCTAGA338750.020.439491
CGCTAAG323550.020.216614
Produced by FastQC (version 0.11.5)