FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129794056
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1662370.12807751381157237No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1477020.1137971988486129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT575650.057.32576
CCGCGTT614600.053.672497
AGACCGC635650.051.758244
CAGACCG676050.049.3181653
GTGCTCT1383200.045.17847145
GCGTTCT764750.043.0938849
TCGACCG596700.042.419933145
CGCGTTC824800.039.9262778
GACCGCG914350.036.240215
CTCGCTA469350.034.4662551
TCGCTAT484250.032.671542
CGCTATG514750.030.7318273
ACCGCTA185750.028.848942
TCAGACC1362650.025.6345772
CTCAGAC1668400.023.9893021
CGGTGCT901300.023.740517145
CGATCCT953250.023.715254145
CTTCGAC788150.022.910053145
CCGCTAA259550.022.5593623
AACGCTA719900.020.251509115-119
Produced by FastQC (version 0.11.5)