FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129794056
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1306140.10063172692592333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG166450.025.1307493
CAATCGC234550.024.6984541
GCGACAT355800.022.972457
TGCGACA355350.022.685736
AGGGCGA753600.021.8752444
CGGAGGG878500.018.9835781
GGCGATC262200.017.3816322
CGGCCGC2579500.017.201605145
CGACATC502950.016.462048
CGTTCTT714600.016.036488145
AATCGCC366050.015.7205292
GGGCGAT290400.015.6582061
ACGCCCG757150.015.5464286
GGGGACC3008550.015.460203145
TGGCGCG816300.015.4594144
GGGCGAG1114250.015.3089015
GCGAGGC1078300.015.235327
CTGCGAC549000.015.1379965
GGCGAGG1155450.014.8817386
CGGTGGC1510650.014.7949131
Produced by FastQC (version 0.11.5)