FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123438986
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1758260.1424396016992557No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1756040.14225975576306174No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1534580.12431890845247223No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA1463950.1185970532842841No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1358390.11004546002994547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT627150.057.2265666
AGACCGC630800.056.59464
CCGCGTT676800.053.0565347
CAGACCG706200.050.992173
GCGTTCT754200.047.5020269
GTGCTCT1306250.044.978092145
TCGACCG421000.038.850143145
CGCGTTC1006100.035.470368
CTCGCTA748250.033.890191
GACCGCG1116500.032.2867555
TCGCTAT829250.030.5379922
CGCTATG834250.030.1678433
TCAGACC1308850.028.5834852
CTCAGAC1630300.026.12151
CGATCCT1380300.023.28633145
ACCGCTA296450.023.0404472
CCGCTAA325250.021.7768573
CGCTAAG347050.019.8790284
GCTATGT1282650.019.8785274
CTTCGAC534500.019.83868145
Produced by FastQC (version 0.11.5)