FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116241316
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG200100.024.8035583
CAATCGC264950.023.0110861
AGGGCGA697950.022.773924
TACCGTA163600.020.7538599
CGGAGGG808200.020.1402261
TGCGACA315900.018.993866
GCGACAT323850.018.8985337
GGGCGAT257100.018.6018981
GGCGATC262350.018.2675192
GAGGGCG993250.016.2193053
CGGCCGC2424400.016.133844145
CTACCGT205000.016.1002628
TGGCGCG881800.016.003724
CGGTGGC1399950.015.7210581
GCGAGGC1041800.015.0145387
AATCGCC409550.014.85113052
GGGCGAG1112900.014.6688795
GGCGAGG1136550.014.3246946
ACTACCG241350.014.2510967
ACGCCCG632800.014.177926
Produced by FastQC (version 0.11.5)