FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121505963
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1550710.12762418911078463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1423650.025.6149371
TGCGACA408150.024.687866
GCGACAT413400.024.4040977
TGGCGCG948150.023.8334644
GTGGCGC1255600.019.5514143
CGACATC538500.019.0901898
GGGCGAT253000.019.0219141
GGCGCGT1050650.018.9519885
CAATCGC202250.018.9236031
TCAATCG139300.018.1725483
CTGCGAC573250.017.8145375
GGTGGCG1690600.017.6926782
GCTGCGA590100.017.2012234
GGCGATC287150.017.0312582
GGGGACC4194800.016.571667145
AGGGCGA653300.015.735654
CGTGCCT1408400.015.3347079
GCGTGCC1332050.014.7165138
ATATCGG227800.014.209884145
CGATCTG371400.013.6318594
Produced by FastQC (version 0.11.5)