FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139011259
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1524860.10969327311825873No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1489350.10713880377128301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG493950.049.927364145
ACCGCGT406200.048.6935046
CCGCGTT466300.042.3493657
AGACCGC464850.042.316974
CAGACCG654200.040.7860533
CTCGCTA526700.032.8211941
TCGCTAT519200.032.527532
GTGCTCT1356700.032.412663145
GCGTTCT616400.032.0364049
CGCTATG544850.030.6569943
CGCGTTC662750.029.6613928
GACCGCG711000.027.9354255
AGACCGT274650.024.8788094
CTTCGAC792750.023.793377145
CCGCTAA320900.023.3767323
ACCGCTA327300.022.9674112
CGATCCT1186600.022.85545145
CGCTAAG341050.021.2300934
TCAGACC1379950.020.7096142
GTCGCGT74100.020.5512751
Produced by FastQC (version 0.11.5)