FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139011259
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC348300.022.1081
TGCGACA357700.021.048666
GCGACAT366200.020.9625747
AGGGCGA800850.019.1148324
TCAATCG264650.019.1033973
TGGCGCG941600.018.462434
CTCGCGT259600.017.056098
TCGCGTC269450.016.4563679
GTGGCGC1297700.016.210713
CGGTGGC1985500.015.4639321
AATCGCC509950.015.2172862
CGACATC525250.014.6759318
CGGCCGC3154650.014.272691145
ATATCGG273250.013.889008145
GGGGACC4051750.013.79946145
GCGAGGC1104600.013.6845037
GGCGATC363450.013.43479252
GGGCGAG1191150.013.152795
CTGCGAC603700.013.012925
ACGCCCG1032250.012.5516776
Produced by FastQC (version 0.11.5)