FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113144369
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCC1332980.11781231463670984No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1247460.11025382977742357No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG1237110.10933906927352258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT384400.044.089386
TCGACCG529750.040.862045145
CCGCGTT420700.040.1934057
AGACCGC419650.039.8030744
CAGACCG457750.037.1227653
CTCGCTA375450.032.375331
GCGTTCT553650.030.5414149
ACCGCTA183500.030.3187312
TCGCTAT394100.030.2926042
GTGCTCT1035350.028.882818145
CGCTATG408950.028.7563063
CTTCGAC748850.026.831804145
CTCGCGT1014050.024.1333248
TCGCGTC1021450.023.9647889
CCGCTAA256800.023.4860153
CGGTGCT644850.023.146044145
GTCGGCT278450.021.8735921
TTCGGCG363950.021.7005187
CGCGTTC782600.021.4420138
CGATCCT817650.020.987246145
Produced by FastQC (version 0.11.5)