FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113144369
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA752850.026.7452664
CAATCGC296500.025.519711
TCGCGTC280600.023.3426199
CTCGCGT280550.023.2545018
TCAATCG208000.022.5595323
GCGACAT352100.021.2127277
TGCGACA349400.021.1913436
TACCGTA149050.018.0641739
GGGCGAG1184700.017.4002885
AATCGCC440100.017.2216572
GGGCGAT357450.017.1763211
GCGAGGC1151500.017.0420557
GGCGATC374750.016.5632822
GGCGAGG1270000.016.2417856
CGACATC499450.015.3302578
TGGCGCG893650.015.093354
CTACCGT191500.014.4654438
CAACCCG555750.014.4177659
ACGCCCG1149950.014.0707246
CGATCTG459050.014.0569324
Produced by FastQC (version 0.11.5)