FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135334558
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1636930.12095432417195318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA968450.030.2401814
TGGCGCG1259600.023.7725124
TGCGACA475950.023.7216246
CTCGCGT303400.023.1518258
TCGCGTC308300.022.8672319
GCGACAT509450.022.3876767
CAATCGC324250.021.6123941
TCAATCG221550.020.6667273
GGGCGAT379050.020.5115641
GGGCGAG1555550.019.7135895
GCGAGGC1505750.019.517447
GGCGATC410550.019.3870812
GTGGCGC1656400.019.1763043
GGCGAGG1607700.018.994516
TACCGTA161000.018.8005499
CGACATC617650.018.764738
CGGTGGC2233250.018.2251221
CGGAGGG1900600.016.8350731
GCTGCGA697250.016.4309024
GGGGACC4927250.016.245491145
Produced by FastQC (version 0.11.5)