FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116470600
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1784440.15320947947378996No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1601220.13747847096177063No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1485230.1275197345939662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT416350.052.474356
CCGCGTT448100.048.842797
TCGACCG799800.047.599613145
AGACCGC459550.047.1238864
CAGACCG506250.043.338363
ACCGCTA231600.040.505112
GCGTTCT601000.036.262019
CTCGCTA386900.035.8362921
GTGCTCT1103450.035.095345145
TCGCTAT397950.034.035842
CCGCTAA301650.033.49683
CGCTATG417450.032.3917663
TCGCGTC294600.030.4983029
CGCTAAG335600.029.569174
CTCGCGT309700.028.847828
CTTCGAC1057050.027.55854145
GTCGCGT53250.025.1186981
CGGTGCT669550.022.913849145
CACCGCT456950.021.6039471
CGCGTTC1006150.021.5857418
Produced by FastQC (version 0.11.5)