FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116470600
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1459700.12532776511840754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA904050.039.554824
CAATCGC356050.034.596621
CGGAGGG1069350.034.0169531
TCAATCG237950.033.03053
GGGCGAT253450.028.9040361
GGCGATC271700.027.2267512
GCGACAT421600.027.2125027
TGCGACA420450.027.1018186
GGGCGAG1393700.026.5386665
GAGGGCG1386200.026.2647423
GCGAGGC1362350.026.1436677
GGCGAGG1471950.025.1826676
CTACCGT241200.024.467818
AATCGCC525750.023.4670932
TACCGTG270550.021.7895289
ACTACCG275700.021.5938077
CGACATC560750.020.6909458
CGAGGCC1770300.020.4559178
GCTGCGA590550.019.3650884
GGTCACG747400.018.713182145
Produced by FastQC (version 0.11.5)