FastQCFastQC Report
Fri 22 Apr 2022
EGAF00005788116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111289991
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA1692250.15205769942060648No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1556760.1398831993795381No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1423020.12786594618378574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT411600.056.785046
TCGACCG547550.054.56925145
CCGCGTT454150.051.5078857
AGACCGC451150.051.4644054
CAGACCG496750.047.397163
CTCGCTA434300.040.1807751
GCGTTCT594050.039.3510789
ACCGCTA174300.038.6760182
TCGCTAT457800.037.612232
GTGCTCT1105450.035.087368145
CGCTATG494100.034.9822043
CCGCTAA250450.028.8516463
CGCGTTC822950.028.3059758
CGATCCT1045000.026.438042145
CTCGCGT159700.025.9521798
CGCTAAG276450.025.7131524
GACCGCG927600.025.3799295
TCGCGTC165250.025.077629
CTTCGAC763750.024.682552145
TCAGACC1115600.022.3673382
Produced by FastQC (version 0.11.5)