FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111289991
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA753300.033.6724054
CAATCGC313500.029.652331
CGGAGGG933600.028.1244871
TCAATCG212250.027.8660623
TGCGACA353600.026.2316686
GCGACAT370100.025.1153987
TACCGTA154500.022.2702799
GAGGGCG1176400.021.9293963
GCGAGGC1177400.021.5840077
GGGCGAG1225000.021.574935
AATCGCC449400.020.5687562
GGCGAGG1295100.020.4405176
GGGCGAT248950.019.897151
GGCGATC261200.019.64942
TGGCGCG1032350.019.5892734
CGGTGGC1570000.019.3833331
CGACATC499950.018.8933538
CTACCGT201050.017.5369118
CTGCGAC543350.017.4699575
GCTGCGA547950.017.1936424
Produced by FastQC (version 0.11.5)