FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112931057
Sequences flagged as poor quality0
Sequence length35-151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT3115900.2759117007113464No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2342540.20743098154124245No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2114490.18723724510964243No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2059200.18234133768888747No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1890120.16736937120848874No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1887020.16709486744642796No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1882710.1667132186675628No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1812660.16051031914099592No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1777080.15735972434934353No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1684660.1491759702559058No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1660790.1470622912880378No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1582310.1401129186278669No Hit
CTGGAGGCGACAGCGAGGGCTGTCTGCCGCGTCAGAGGACCCCGCCGGCC1474420.13055930221214523No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1453690.12872366899036464No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1400550.12401814321103893No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1391180.12318843345281007No Hit
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG1281620.11348693920397823No Hit
CCACACACGACCGGTCGGAGGCAGAACGGCAGCCCCTCGGCGGCCGGCCG1277360.11310971790514633No Hit
CCCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGG1267730.11225698525074462No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1213450.10745051292666108No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1172910.10386071211571145No Hit
CTCCGTCTTCGCGGGCGGCGGCGGCGCCGCCCTCCCCGTCTCTCTCAGCC1148430.10169301789143795No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1138890.10084825470109608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC577650.047.881144
ACCGCGT587600.047.779846
GTGCTCT1076000.044.39011145
ACCGCTA302450.044.2085382
CAGACCG643600.043.6750953
CCGCGTT644000.043.6159747
GCGTTCT726950.038.194479
CTCGCTA1075900.037.9386251
TCGACCG1019100.035.563972145
CCGCTAA400850.034.984573
TCGCTAT1214750.033.967982
GTCTCGC868550.032.5314874
CGCTATG1333100.031.1342183
GTCGCGT105200.030.2639391
CGCTAAG473500.029.7315464
GGTCTCG1092900.027.2442233
GGGGTCT1468900.025.8798641
CTTCGAC1251050.025.632885145
GCTATGT1633700.025.547184
TCTCGCT1110150.025.4853065
Produced by FastQC (version 0.11.5)