FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112931057
Sequences flagged as poor quality0
Sequence length35-151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1835270.16251242561202628No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1574760.13944436914284794No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1368050.121140281189434No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1273140.11273603859034101No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1267580.1122437028106449No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA1220760.10809781050752053No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1138950.10085356767713598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG246100.034.4304243
AGGGCGA1017500.033.408344
CAATCGC369450.030.5962141
GGGCGAT328350.027.9113791
GGCGATC336300.027.2986092
CGGTGGC2690450.025.9894331
TGGCGCG1912400.025.432184
TGCGACA526550.023.0006666
GCGACAT535950.022.877427
CGGAGGG1583250.022.426031
GTGGCGC2289650.022.2042523
AATCGCC532950.021.5117762
GATCAAT424300.020.935441
GGGCGAG1730350.020.4079075
ATCAATC450800.020.2816622
GGCGAGG1765450.020.023716
ACGCCCG1158150.019.266956
GCGAGGC1823100.018.481247
GAGGGCG1932900.018.2990323
CGTGCCT2833800.018.0729929
Produced by FastQC (version 0.11.5)