FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103843170
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1759970.16948346241741272No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1645320.15844277481128513No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1542670.14855767596463013No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1371840.1321069069829051No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1147520.11050510110583105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT405700.058.4188586
AGACCGC429200.055.0732464
CCGCGTT443550.053.491427
CAGACCG471850.050.7369883
TCGACCG582100.049.996487145
GCGTTCT592800.039.9067579
CTCGCTA570300.038.454351
TCGCTAT585150.036.7085152
GTGCTCT999300.036.49006145
CGCTATG621300.034.5320363
CGCGTTC698150.033.767418
GACCGCG722750.032.9317935
CGATCCT1188700.025.12477145
TCAGACC1070650.023.6744172
CTCAGAC1258100.023.2914161
CTTCGAC809150.023.263266145
GAACGTA177950.021.1637695
CCGCTAA277000.020.2459853
CGTATCG102300.020.2262383
GCTATGT1115300.019.4743084
Produced by FastQC (version 0.11.5)