FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103843170
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1486950.1431918921581458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC231150.024.4480551
TGCGACA361550.023.9841376
GCGACAT367200.023.8740377
CGGTGGC1316300.023.2111
TGGCGCG840250.022.9399624
TCAATCG160350.020.92463
CTACCGT209450.020.5795048
AGGGCGA626000.019.9702194
GTGGCGC1095900.019.284333
GGGCGAT228600.018.5618631
CGACATC479100.018.5360978
GGCGATC239750.018.2536562
ACTACCG250500.017.409537
CGGAGGG798450.016.6581271
GGGGACC3399150.016.16868145
CTGCGAC545000.016.131935
GGCGCGT1110900.015.9881985
GCTGCGA554450.015.9238234
TACCGTA281600.015.7116429
GGTGGCG1664550.015.4813692
Produced by FastQC (version 0.11.5)