FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171451685
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1146400.033.054134
TGGCGCG1890350.026.0152384
CGGTGGC2646750.025.5874351
TCAATCG323150.025.5535053
TGCGACA476950.023.7490836
CAATCGC444850.023.5906431
GCGACAT506700.022.6935947
CGGAGGG1824750.021.7006761
GTGGCGC2392550.021.6230183
GGGCGAG1853600.021.2594075
GCGAGGC1893150.020.1030677
GGCGAGG2015400.019.6094676
TACCGTA272150.019.58259
GAGGGCG2000700.019.4539363
TCGCATA142150.018.83617
TCGCGTC262500.018.1703979
GTAATCG161950.017.6378383
CTCGCGT271450.017.4773778
GGGGACC7671200.017.272713145
GGTGGCG3381450.017.2718682
Produced by FastQC (version 0.11.5)