FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118873302
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1220180.10264541991102426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC220650.028.4083181
CGGTGGC1605050.026.983191
TCAATCG147500.025.2988433
TGGCGCG1021350.024.876194
GTGGCGC1360350.023.1322693
AGGGCGA734000.022.7666384
GGGGACC4134500.019.29063145
GGTGGCG2018400.019.197172
GCGACAT289150.019.06757
CGGAGGG927300.018.6301361
GGCGCGT1254650.018.61995
AATCGCC345650.018.1882672
TGCGACA315000.017.3041216
CTAACGC245900.016.683905145
GGGCGAG1042300.016.1090475
GCGAGGC1060550.015.5308687
ACGCCCG598450.015.1315216
ATATCGG210600.014.679543145
GGCGAGG1158750.014.6791736
GGCGATC251400.014.4712152
Produced by FastQC (version 0.11.5)