FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99061682
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1378290.139134524285586No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1353410.13662295780521877No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1275050.12871273475853157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT322950.056.7029466
TCGACCG518950.056.248444145
AGACCGC355500.051.2993054
CCGCGTT368450.049.71917
CAGACCG398550.046.5368273
CTCGCTA354150.039.7875141
GCGTTCT469350.039.030589
TCGCTAT374600.036.7964822
CGCTATG396800.034.4861263
CGCGTTC564600.032.2513548
ACCGCTA135800.031.0846672
GTGCTCT1005200.029.09123145
GACCGCG642050.028.7261355
CTTCGAC708900.024.57793145
CCGCTAA185850.024.5316643
CGATCCT928100.024.4351145
CGCTAAG210500.021.344244
GTCGCGT55250.020.7519041
TCAGACC968550.020.4482362
CCCGTAT177100.019.753011
Produced by FastQC (version 0.11.5)