FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99061682
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG159150.027.868493
AGGGCGA625600.027.0393964
CAATCGC230800.025.4086761
TGCGACA346400.024.0849276
GCGACAT366550.023.2384327
CGGAGGG792300.021.9382061
TGGCGCG799850.021.5783964
GGCGATC225900.019.7022112
GGGCGAT230950.019.21571
TACCGTA128150.019.0698669
CGGTGGC1268650.018.849141
GCGAGGC907750.018.474597
GGGCGAG952650.018.3659845
CGACATC493550.017.5990648
CGGCCGC2384750.017.537472145
GGCGAGG1001000.017.4011616
AATCGCC338400.017.3893782
GAGGGCG990950.017.3796223
GTGGCGC1062600.016.891193
GCTGCGA511500.016.7953824
Produced by FastQC (version 0.11.5)