FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110176334
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1796760.1630803943794318No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1185990.10764471433583912No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1123540.10197652791751086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT318300.050.491766
TCGACCG529600.045.821198145
CCGCGTT353600.045.379337
AGACCGC366250.043.79394
GCGTTCT613100.041.1052679
CAGACCG425450.038.5272563
GTGCTCT1127150.037.258778145
CTCGCTA379050.036.422341
CGCGTTC496200.032.286468
TCGCTAT429750.031.8890172
CGCTATG428150.031.7392063
GACCGCG527100.030.5753965
ACTGCGT331500.028.1584856
GACTGCG391300.023.6916585
CTTCGAC745250.022.817772145
CTCAGAC1448200.021.8035551
CGATCCT853800.021.74089145
TGCGTTC456550.021.1047138
CGGTGCT710550.019.854202145
CGTATCG125150.019.8352813
Produced by FastQC (version 0.11.5)