FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110176334
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1704140.15467387034315375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA333650.022.6331526
GCGACAT334550.022.3800097
CAATCGC231250.021.0983331
TACCGTA189500.020.4338449
CTACCGT207850.018.5991348
TCAATCG181550.017.9030673
AGGGCGA592800.017.8169254
GGCGATC262700.017.1743072
CGACATC459350.016.5936898
CGGTGGC1254900.016.4911
GGGCGAT274850.016.1943171
TGGCGCG739350.015.17328554
ACTACCG258850.015.10858257
CTCGCGT154850.015.0371548
TCGCGTC152800.015.0282249
CTGCGAC521800.014.6693545
ACGCCCG719550.014.151036
GCTGCGA553450.013.815624
CGATCTG355800.013.2957764
GGGGACC2639150.013.252562145
Produced by FastQC (version 0.11.5)