FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89248897
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2243390.2513633305742703No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1260060.14118493811749852No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1019390.11421877852451218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT506950.059.1208346
AGACCGC529300.056.5913124
CCGCGTT542850.055.186857
CAGACCG563750.053.491383
GTGCTCT1047300.052.259937145
GCGTTCT664150.045.174069
TCGACCG371150.039.362076145
CGCGTTC789650.037.8506628
CTCGCTA350850.037.560841
GACCGCG830900.036.1848035
TCGCTAT380900.034.1268732
CGCTATG403200.032.198673
ACCGCTA122200.030.9095082
TCAGACC1064850.029.4801752
CTCAGAC1247000.027.4560241
CTTCGAC529650.026.821653145
CGGTGCT778200.025.014366145
CCGCTAA177550.023.9942553
CGATCCT785150.022.76354145
CGCTAAG197250.020.9579224
Produced by FastQC (version 0.11.5)