FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89248897
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG151100.028.859153
CAATCGC226400.028.5130121
AGGGCGA550850.022.5946144
TGGCGCG720750.019.5791534
AATCGCC337550.019.2853432
TACCGTA131250.018.933019
GCGACAT249850.018.6206557
CGGTGGC1202900.018.50421
CGGAGGG736600.017.9647241
TGCGACA262100.017.5566376
GGGCGAT201050.017.0794931
ACGCCCG653600.016.9105346
CGTTCTT592050.016.88429145
GGCGATC208600.016.4567852
GCGAGGC788200.015.6433067
GTGGCGC964350.015.5824823
GGGCGAG849900.015.0537535
GGCGCGT901300.014.8651765
AGGGATC626500.014.8148522
GGCGAGG864000.014.8081166
Produced by FastQC (version 0.11.5)