FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128957696
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2763210.2142725937039074No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2521090.19549744437121455No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2005760.1555362775712122No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1920620.14893411247049573No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1905460.14775853315493478No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1876090.14548104209305973No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1749460.13566154283649734No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1694990.1314376770503096No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1562900.12119478313260187No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1528850.1185543823611737No Hit
CTCCGTCTTCGCGGGCGGCGGCGGCGCCGCCCTCCCCGTCTCTCTCAGCC1510820.11715624944167737No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1313090.10182331421305789No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1300320.10083306699276016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT428100.048.9746486
AGACCGC432800.047.854334
TCGACCG1052000.047.705452145
CTCGCTA776500.045.21441
ACCGCTA299300.044.2120482
CCGCGTT477350.044.0127377
TCGCTAT850600.041.4873922
CGCTATG929750.037.9123653
CAGACCG596500.035.3871963
CCGCTAA413750.034.131593
GCGTTCT645150.032.324769
GTGCTCT1165650.030.66308145
CGCTAAG468050.030.25144
CTTCGAC1369300.029.599977145
CGATCCT1743750.027.019861145
GCTATGT1433300.024.9175764
GTCGCGT118450.024.6201731
CACCGCT579950.023.571621
CTATGTT1572550.022.841325
GTCTCGC706600.022.6727774
Produced by FastQC (version 0.11.5)